Hotspot

ndhF

SSC · 115958-118189 · 2232 bp

Back to Hevea

Pi 0.000896
MarkerSeek score 31.40
Primer available yes
Label ndhF

Feature Metadata

label_name
ndhF
feature_type
gene
region
SSC
length_bp
2232
variable_sites
6
pi
0.000896
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
31.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF_p1 CGTATCCACCCAGTTTGTCA TCCGTTCCAAAATGCACTAGT 50.0 42.9 57.5 57.9 2925-2927 bp 1.0 68.2
ndhF_p2 ACGTATCCACCCAGTTTGTC TCCGTTCCAAAATGCACTAGT 50.0 42.9 57.5 57.9 2926-2928 bp 1.0 68.1
ndhF_p3 TCCACCCAGTTTGTCAACTT TCCGTTCCAAAATGCACTAGT 45.0 42.9 57.2 57.9 2921-2923 bp 1.0 65.3
ndhF_p4 CGTATCCACCCAGTTTGTCA CCGTTCCAAAATGCACTAGT 50.0 45.0 57.5 56.3 2924-2926 bp 1.0 64.0
ndhF_p5 ACGTATCCACCCAGTTTGTC CCGTTCCAAAATGCACTAGT 50.0 45.0 57.5 56.3 2925-2927 bp 1.0 63.9