Hotspot

clpP

LSC · 74392-76462 · 2071 bp

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Pi 0.000354
MarkerSeek score 49.00
Primer available yes
Label clpP

Feature Metadata

label_name
clpP
feature_type
gene
region
LSC
length_bp
2071
variable_sites
2
pi
0.000354
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
49.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
clpP_p1 GCGATTTGCAGGAAAATTTCTG AAACTTATCGAATCCACGCA 40.9 40.0 57.4 55.5 2210-2218 bp 1.0 40.4
clpP_p2 GCGATTTGCAGGAAAATTTCTG AACTTATCGAATCCACGCAA 40.9 40.0 57.4 55.5 2209-2217 bp 1.0 40.4
clpP_p3 GCGATTTGCAGGAAAATTTCTG ACTTATCGAATCCACGCAAA 40.9 40.0 57.4 55.5 2208-2216 bp 1.0 40.4
clpP_p4 GCGATTTGCAGGAAAATTTCTG AACTTATCGAATCCACGCAAA 40.9 38.1 57.4 56.3 2209-2217 bp 1.0 40.4
clpP_p5 GCGATTTGCAGGAAAATTTCTG ACTTATCGAATCCACGCAAAA 40.9 38.1 57.4 56.3 2208-2216 bp 1.0 40.4