Hotspot

atpF

LSC · 29582-30136 · 555 bp

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Pi 0.000961
MarkerSeek score 27.40
Primer available yes
Label atpF

Feature Metadata

label_name
atpF
feature_type
gene
region
LSC
length_bp
555
variable_sites
1
pi
0.000961
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
27.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF_p1 AGTCTATAAGAAAAGAGGAGATCA TAATGCCTACAGTAGAAGGA 33.3 40.0 54.3 52.4 600 bp 1.0 40.1
atpF_p2 AGTCTATAAGAAAAGAGGAGATCA ATAATGCCTACAGTAGAAGGA 33.3 38.1 54.3 53.2 601 bp 1.0 40.1
atpF_p3 AGTCTATAAGAAAAGAGGAGATCA AATAATGCCTACAGTAGAAGGA 33.3 36.4 54.3 54.0 602 bp 1.0 40.1
atpF_p4 GTCTATAAGAAAAGAGGAGATCA TAATGCCTACAGTAGAAGGA 34.8 40.0 52.8 52.4 599 bp 1.0 40.1
atpF_p5 AGTCTATAAGAAAAGAGGAGATCAT TAATGCCTACAGTAGAAGGA 32.0 40.0 54.8 52.4 600 bp 1.0 40.1