Hotspot

trnV(GAC)-rps12

IRa · 144458-146351 · 1894 bp

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Pi 0.00102
MarkerSeek score 43.50
Primer available yes
Label trnV(GAC)-rps12

Feature Metadata

label_name
trnV(GAC)-rps12
feature_type
igs
region
IRa
length_bp
1894
variable_sites
5
pi
0.00102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV(GAC)-rps12_p1 GATGACTTCCACCACGTCAA TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1986-1990 bp 1.0 77.1
trnV(GAC)-rps12_p2 AACTGATGACTTCCACCACG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1990-1994 bp 1.0 77.1
trnV(GAC)-rps12_p3 ATGACTTCCACCACGTCAAG TGGCAACTTTACGTAAGGCA 50.0 45.0 57.8 57.7 1985-1989 bp 1.0 77.1
trnV(GAC)-rps12_p4 CTGATGACTTCCACCACGTC TGGCAACTTTACGTAAGGCA 55.0 45.0 58.3 57.7 1988-1992 bp 1.0 76.1
trnV(GAC)-rps12_p5 GTCAAGGTGACACTCTACCG TGGCAACTTTACGTAAGGCA 55.0 45.0 57.7 57.7 1971-1975 bp 1.0 75.6