Hotspot

rpl32-trnL(TAG)

SSC · 119343-120165 · 823 bp

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Pi 0.00186
MarkerSeek score 51.00
Primer available yes
Label rpl32-trnL(TAG)

Feature Metadata

label_name
rpl32-trnL(TAG)
feature_type
igs
region
SSC
length_bp
823
variable_sites
4
pi
0.00186
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL(TAG)_p1 TGAAAGCTTTTTCGTTAGCGA AGCGTGTCTACCGATTTCAC 38.1 50.0 56.8 58.0 928-929 bp 1.0 62.6
rpl32-trnL(TAG)_p2 TGAAAGCTTTTTCGTTAGCGA CAGCGTGTCTACCGATTTCA 38.1 50.0 56.8 58.0 929-930 bp 1.0 62.5
rpl32-trnL(TAG)_p3 TGAAAGCTTTTTCGTTAGCGA TCCTAAGAGCAGCGTGTCTA 38.1 50.0 56.8 57.9 938-939 bp 1.0 61.6
rpl32-trnL(TAG)_p4 TGAAAGCTTTTTCGTTAGCGA CCTAAGAGCAGCGTGTCTAC 38.1 55.0 56.8 57.8 937-938 bp 1.0 61.0
rpl32-trnL(TAG)_p5 TGAAAGCTTTTTCGTTAGCGA CTAAGAGCAGCGTGTCTACC 38.1 55.0 56.8 57.8 936-937 bp 1.0 61.0