Hotspot

rps12-trnV(GAC)

IRb · 103884-105777 · 1894 bp

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Pi 0.00102
MarkerSeek score 43.50
Primer available yes
Label rps12-trnV(GAC)

Feature Metadata

label_name
rps12-trnV(GAC)
feature_type
igs
region
IRb
length_bp
1894
variable_sites
5
pi
0.00102
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
43.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV(GAC)_p1 TGGCAACTTTACGTAAGGCA GATGACTTCCACCACGTCAA 45.0 50.0 57.7 57.8 1986-1990 bp 1.0 77.1
rps12-trnV(GAC)_p2 TGGCAACTTTACGTAAGGCA AACTGATGACTTCCACCACG 45.0 50.0 57.7 57.8 1990-1994 bp 1.0 77.1
rps12-trnV(GAC)_p3 TGGCAACTTTACGTAAGGCA ATGACTTCCACCACGTCAAG 45.0 50.0 57.7 57.8 1985-1989 bp 1.0 77.1
rps12-trnV(GAC)_p4 TGGCAACTTTACGTAAGGCA CTGATGACTTCCACCACGTC 45.0 55.0 57.7 58.3 1988-1992 bp 1.0 76.1
rps12-trnV(GAC)_p5 TGGCAACTTTACGTAAGGCA GTCAAGGTGACACTCTACCG 45.0 55.0 57.7 57.7 1971-1975 bp 1.0 75.6