Hotspot

rpl16-rps3

LSC · 86347-87711 · 1365 bp

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Pi 0.00137
MarkerSeek score 50.60
Primer available yes
Label rpl16-rps3

Feature Metadata

label_name
rpl16-rps3
feature_type
igs
region
LSC
length_bp
1365
variable_sites
4
pi
0.00137
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl16-rps3_p1 GAATCGGGTTCTTTTGGGGT TCCTCTACAAACCATTGGAGC 50.0 47.6 58.1 57.6 1501-1510 bp 1.0 68.9
rpl16-rps3_p2 GAATCGGGTTCTTTTGGGGT CCTCTACAAACCATTGGAGCT 50.0 47.6 58.1 57.6 1500-1509 bp 1.0 68.9
rpl16-rps3_p3 AATCGGGTTCTTTTGGGGTT TCCTCTACAAACCATTGGAGC 45.0 47.6 57.6 57.6 1500-1509 bp 1.0 66.4
rpl16-rps3_p4 AATCGGGTTCTTTTGGGGTT CCTCTACAAACCATTGGAGCT 45.0 47.6 57.6 57.6 1499-1508 bp 1.0 66.4
rpl16-rps3_p5 GTTCAAGTGCCTGAAGAGCA TCCTCTACAAACCATTGGAGC 50.0 47.6 58.4 57.6 1589-1598 bp 1.0 66.3