Hotspot

petB-petD

LSC · 80800-81820 · 1021 bp

Pi 0

MarkerSeek score 48.40

Primer available yes

Label petB-petD

Feature Metadata

label_name: petB-petD
feature_type: igs
region: LSC
length_bp: 1021
variable_sites: 0
pi: 0
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petB-petD_p1	AGCAGGGTATTTCAGGTCCT	ATGACCCATTCCCTTAGCCA	50.0	50.0	58.0	58.4	1144-1161 bp	1.0	77.9
petB-petD_p2	AGCAGGGTATTTCAGGTCCT	TGACCCATTCCCTTAGCCAA	50.0	50.0	58.0	58.6	1143-1160 bp	1.0	76.0
petB-petD_p3	AGCAGGGTATTTCAGGTCCT	GACCCATTCCCTTAGCCAAT	50.0	50.0	58.0	57.2	1142-1159 bp	1.0	75.0
petB-petD_p4	AGCAGGGTATTTCAGGTCCT	ATCCATCCTCCGAAAGAACC	50.0	50.0	58.0	57.0	1056-1073 bp	1.0	73.0
petB-petD_p5	AGCAGGGTATTTCAGGTCCT	GACCCATTCCCTTAGCCAATT	50.0	47.6	58.0	58.0	1142-1159 bp	1.0	72.8