Hotspot

rpl20-rps12

LSC · 73258-74067 · 810 bp

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Pi 0.000658
MarkerSeek score 50.30
Primer available yes
Label rpl20-rps12

Feature Metadata

label_name
rpl20-rps12
feature_type
igs
region
LSC
length_bp
810
variable_sites
1
pi
0.000658
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl20-rps12_p1 TTTTGTTCTACGCCTCCGAG CGCCGAGGAACATGTACTAG 50.0 55.0 57.9 57.9 883-885 bp 1.0 78.8
rpl20-rps12_p2 GTAAGTCTTGAATGGGCCCC CGCCGAGGAACATGTACTAG 55.0 55.0 58.2 57.9 930-932 bp 1.0 77.5
rpl20-rps12_p3 TTTTGTTCTACGCCTCCGAG AAATGTCACCAAATCCCCCG 50.0 50.0 57.9 58.4 923-925 bp 1.0 76.2
rpl20-rps12_p4 TTTTGTTCTACGCCTCCGAG GCCGAGGAACATGTACTAGG 50.0 55.0 57.9 57.5 882-884 bp 1.0 75.7
rpl20-rps12_p5 TTTTTGTTCTACGCCTCCGA CGCCGAGGAACATGTACTAG 45.0 55.0 57.5 57.9 884-886 bp 1.0 75.2