Hotspot

psaJ-rpl33

LSC · 71227-71695 · 469 bp

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Pi 0.00114
MarkerSeek score 48.50
Primer available yes
Label psaJ-rpl33

Feature Metadata

label_name
psaJ-rpl33
feature_type
igs
region
LSC
length_bp
469
variable_sites
1
pi
0.00114
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaJ-rpl33_p1 TTTTCCCAGATGCGTTGACA ACACTGTTTCGAGCACAAGT 45.0 45.0 58.0 58.0 573-578 bp 1.0 80.4
psaJ-rpl33_p2 TTCCCAGATGCGTTGACATT ACACTGTTTCGAGCACAAGT 45.0 45.0 57.8 58.0 571-576 bp 1.0 79.1
psaJ-rpl33_p3 TTTCCCAGATGCGTTGACAT ACACTGTTTCGAGCACAAGT 45.0 45.0 57.8 58.0 572-577 bp 1.0 79.1
psaJ-rpl33_p4 CAGATGCGTTGACATTCCCT ACACTGTTTCGAGCACAAGT 50.0 45.0 58.3 58.0 567-572 bp 1.0 78.6
psaJ-rpl33_p5 TCCCAGATGCGTTGACATTC ACACTGTTTCGAGCACAAGT 50.0 45.0 58.3 58.0 570-575 bp 1.0 78.5