Hotspot

trnP(TGG)-psaJ

LSC · 70671-71091 · 421 bp

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Pi 0
MarkerSeek score 48.40
Primer available yes
Label trnP(TGG)-psaJ

Feature Metadata

label_name
trnP(TGG)-psaJ
feature_type
igs
region
LSC
length_bp
421
variable_sites
0
pi
0
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnP(TGG)-psaJ_p1 ACCCGTGACATTTTGTACCC TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 565-615 bp 1.0 82.5
trnP(TGG)-psaJ_p2 CCCGTGACATTTTGTACCCA TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 564-614 bp 1.0 82.5
trnP(TGG)-psaJ_p3 ACAGGATTTGAACCCGTGAC TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 576-626 bp 1.0 82.4
trnP(TGG)-psaJ_p4 CAGGATTTGAACCCGTGACA TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 575-625 bp 1.0 82.4
trnP(TGG)-psaJ_p5 TGACAGGATTTGAACCCGTG TGTCAACGCATCTGGGAAAA 50.0 45.0 58.1 58.0 578-628 bp 1.0 82.3