Hotspot

psbE-petL

LSC · 68470-69832 · 1363 bp

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Pi 0.00103
MarkerSeek score 50.30
Primer available yes
Label psbE-petL

Feature Metadata

label_name
psbE-petL
feature_type
igs
region
LSC
length_bp
1363
variable_sites
3
pi
0.00103
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbE-petL_p1 AGAACGTTCTCCTGTGCTTC GCCGCTAGTAGAAAACCGAA 50.0 50.0 57.8 58.0 1429-1431 bp 1.0 79.0
psbE-petL_p2 AGAACGTTCTCCTGTGCTTC AAGCCGCTAGTAGAAAACCG 50.0 50.0 57.8 58.0 1431-1433 bp 1.0 79.0
psbE-petL_p3 GTTCTCCTGTGCTTCCAGAC GCCGCTAGTAGAAAACCGAA 55.0 50.0 58.2 58.0 1424-1426 bp 1.0 78.7
psbE-petL_p4 GTTCTCCTGTGCTTCCAGAC AAGCCGCTAGTAGAAAACCG 55.0 50.0 58.2 58.0 1426-1428 bp 1.0 78.6
psbE-petL_p5 TCTCCTGTGCTTCCAGACAT GCCGCTAGTAGAAAACCGAA 50.0 50.0 58.4 58.0 1422-1424 bp 1.0 77.5