Hotspot

psaI-ycf4

LSC · 62743-63198 · 456 bp

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Pi 0.000731
MarkerSeek score 47.70
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
456
variable_sites
1
pi
0.000731
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
47.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 ATTTCCGGCAATTGCAATGG GTTTTTCGAGACCCCGCTAT 45.0 50.0 58.0 58.0 571-576 bp 1.0 80.2
psaI-ycf4_p2 GGGCTTAGTATTTCCGGCAA GTTTTTCGAGACCCCGCTAT 50.0 50.0 58.0 58.0 580-585 bp 1.0 80.0
psaI-ycf4_p3 ATTTCCGGCAATTGCAATGG TCGAGACCCCGCTATAAGTT 45.0 50.0 58.0 57.9 566-571 bp 1.0 79.8
psaI-ycf4_p4 ATTTCCGGCAATTGCAATGG TTCGAGACCCCGCTATAAGT 45.0 50.0 58.0 57.9 567-572 bp 1.0 79.8
psaI-ycf4_p5 CCCTCCATTCTTGTGCCTTT GTTTTTCGAGACCCCGCTAT 50.0 50.0 58.1 58.0 603-608 bp 1.0 79.8