Hotspot

ycf1

SSC · 125471-131101 · 5631 bp

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Pi 0.00671

MarkerSeek score 46.80

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5631
variable_sites: 112
pi: 0.00671
unique_haplotype_count: 12
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	TGGGGCGGATTCTAATCTTT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	56.6	58.0	516 bp	1.0	71.0
ycf1_p2	TTGGGGCGGATTCTAATCTT	CCTTTTGGTTCTCCCCGAAA	45.0	50.0	56.6	58.0	517 bp	1.0	71.0
ycf1_p3	TGGGGCGGATTCTAATCTTT	TCTGTCTGAAACCTTGGCAC	45.0	50.0	56.6	58.0	635 bp	1.0	70.8
ycf1_p4	TGGGGCGGATTCTAATCTTT	GTCTGAAACCTTGGCACAGA	45.0	50.0	56.6	58.0	632 bp	1.0	70.7
ycf1_p5	TTGGGGCGGATTCTAATCTT	GTCTGAAACCTTGGCACAGA	45.0	50.0	56.6	58.0	633 bp	1.0	70.7