Hotspot

rpl20

LSC · 70356-70742 · 387 bp

Back to Heuchera

Pi 0.0054

MarkerSeek score 34.10

Primer available yes

Label rpl20

Feature Metadata

label_name: rpl20
feature_type: gene
region: LSC
length_bp: 387
variable_sites: 7
pi: 0.0054
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 34.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl20_p1	GAGTTCATTCTCCGGGGAAC	TCGACCAGTAAAGAGGAAAAGA	55.0	40.9	58.0	56.8	487 bp	1.0	55.2
rpl20_p2	CCTTCCCGGAGTTCATTCTC	TCGACCAGTAAAGAGGAAAAGA	55.0	40.9	57.7	56.8	495 bp	1.0	52.9
rpl20_p3	ACCTTCCCGGAGTTCATTCT	TCGACCAGTAAAGAGGAAAAGA	50.0	40.9	58.3	56.8	496 bp	1.0	52.7
rpl20_p4	GAGTTCATTCTCCGGGGAAC	TCGACCAGTAAAGAGGAAAAGAA	55.0	39.1	58.0	57.5	487 bp	1.0	52.6
rpl20_p5	GAGTTCATTCTCCGGGGAAC	CGACCAGTAAAGAGGAAAAGA	55.0	42.9	58.0	55.3	486 bp	1.0	51.2