Hotspot

rps19-trnH-GUG

LSC · 155445-155454 · 10 bp

Back to Heuchera

Pi 0.0909
MarkerSeek score 58.30
Primer available yes
Label rps19-trnH-GUG

Feature Metadata

label_name
rps19-trnH-GUG
feature_type
igs
region
LSC
length_bp
10
variable_sites
3
pi
0.0909
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps19-trnH-GUG_p1 TGGGTAGAGCCGGATCTAAG CCAAGTGGATAAGGCAGTGG 55.0 55.0 58.0 58.2 412-418 bp 0.75 68.8
rps19-trnH-GUG_p2 GGGCCCCAATTGGTAGAAAA CCAAGTGGATAAGGCAGTGG 50.0 55.0 58.1 58.2 313-319 bp 0.75 68.6
rps19-trnH-GUG_p3 AGAAAAAGACCCACAACCCC CCAAGTGGATAAGGCAGTGG 50.0 55.0 57.9 58.2 299-305 bp 0.75 68.4
rps19-trnH-GUG_p4 GAAAAAGACCCACAACCCCT CCAAGTGGATAAGGCAGTGG 50.0 55.0 57.9 58.2 298-304 bp 0.75 68.4
rps19-trnH-GUG_p5 AAAAGTTTGGGTAGAGCCGG CCAAGTGGATAAGGCAGTGG 50.0 55.0 58.1 58.2 419-425 bp 0.75 68.0