Hotspot

ccsA-ndhD

SSC · 116902-117124 · 223 bp

Back to Heuchera

Pi 0.0341

MarkerSeek score 58.60

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 223
variable_sites: 17
pi: 0.0341
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TGTGGCTTCTATGGGGTTTC	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.5	57.5	368-378 bp	1.0	69.7
ccsA-ndhD_p2	GTGGCTTCTATGGGGTTTCT	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.2	57.5	367-377 bp	1.0	67.3
ccsA-ndhD_p3	GCAATTGTGGCTTCTATGGG	TCAGTTGACAAGGTCGAAACT	50.0	42.9	57.1	57.5	373-383 bp	1.0	66.7
ccsA-ndhD_p4	TTGTGGCTTCTATGGGGTTT	TCAGTTGACAAGGTCGAAACT	45.0	42.9	57.0	57.5	369-379 bp	1.0	65.8
ccsA-ndhD_p5	TGTGGCTTCTATGGGGTTTC	TCAGTTGACAAGGTCGAAAC	50.0	45.0	57.5	55.9	368-378 bp	1.0	65.5