Hotspot

trnR-ACG-trnN-GUU

IRb · 109296-109896 · 601 bp

Back to Heuchera

Pi 0.00368
MarkerSeek score 53.70
Primer available yes
Label trnR-ACG-trnN-GUU

Feature Metadata

label_name
trnR-ACG-trnN-GUU
feature_type
igs
region
IRb
length_bp
601
variable_sites
8
pi
0.00368
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-trnN-GUU_p1 CAGAGGATTAGAGCACGTGG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.8 58.0 735-757 bp 1.0 79.9
trnR-ACG-trnN-GUU_p2 GAACAGATCCAGTGGAGACG CCGTTGTTCCTCAGTAGCTC 55.0 55.0 57.7 58.0 772-794 bp 1.0 79.2
trnR-ACG-trnN-GUU_p3 CAGAGGATTAGAGCACGTGG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.8 58.1 776-798 bp 1.0 79.0
trnR-ACG-trnN-GUU_p4 CAGAGGATTAGAGCACGTGG TGGTCGTAGGTTCGAATCCT 55.0 50.0 57.8 58.2 686-708 bp 1.0 78.8
trnR-ACG-trnN-GUU_p5 GAACAGATCCAGTGGAGACG TGGGTTGAGAACGGGAATTG 55.0 50.0 57.7 58.1 813-835 bp 1.0 78.4