Hotspot

trnP-UGG-psaJ

LSC · 68450-68853 · 404 bp

Back to Heuchera

Pi 0.00679

MarkerSeek score 57.00

Primer available yes

Label trnP-UGG-psaJ

Feature Metadata

label_name: trnP-UGG-psaJ
feature_type: igs
region: LSC
length_bp: 404
variable_sites: 9
pi: 0.00679
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnP-UGG-psaJ_p1	ACCCGTGACATTTTGTACCC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	476-495 bp	1.0	84.4
trnP-UGG-psaJ_p2	CCCGTGACATTTTGTACCCA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	475-494 bp	1.0	84.4
trnP-UGG-psaJ_p3	ACAGGATTTGAACCCGTGAC	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	487-506 bp	1.0	84.3
trnP-UGG-psaJ_p4	CAGGATTTGAACCCGTGACA	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	486-505 bp	1.0	84.3
trnP-UGG-psaJ_p5	TGACAGGATTTGAACCCGTG	GGTGCCACGGAAAGATATGT	50.0	50.0	58.1	58.0	489-508 bp	1.0	84.3