Hotspot

atpB-rbcL

LSC · 56439-57202 · 764 bp

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Pi 0.00497

MarkerSeek score 53.50

Primer available yes

Label atpB-rbcL

Feature Metadata

label_name: atpB-rbcL
feature_type: igs
region: LSC
length_bp: 764
variable_sites: 11
pi: 0.00497
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpB-rbcL_p1	GGGGGAAAAGCTACATCCAG	CACCAGCTTTGAATCCAACAC	55.0	47.6	57.9	58.0	902-924 bp	1.0	74.7
atpB-rbcL_p2	GGGGGAAAAGCTACATCCAG	ACCAGCTTTGAATCCAACACT	55.0	42.9	57.9	58.1	901-923 bp	1.0	74.5
atpB-rbcL_p3	AATTTGAACGATACGCCCCA	CACCAGCTTTGAATCCAACAC	45.0	47.6	57.9	58.0	870-892 bp	1.0	74.4
atpB-rbcL_p4	AATTTGAACGATACGCCCCA	ACCAGCTTTGAATCCAACACT	45.0	42.9	57.9	58.1	869-891 bp	1.0	74.1
atpB-rbcL_p5	AAGCTACATCCAGTACCGGA	CACCAGCTTTGAATCCAACAC	50.0	47.6	57.8	58.0	895-917 bp	1.0	74.0