Hotspot

trnF-GAA-ndhJ

LSC · 49978-50626 · 649 bp

Back to Heuchera

Pi 0.0114

MarkerSeek score 55.30

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 649
variable_sites: 21
pi: 0.0114
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 55.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCTGGTAGA	GTTGGATAGGATGGCCCTTG	55.0	55.0	58.0	58.0	790-814 bp	1.0	84.0
trnF-GAA-ndhJ_p2	CCTCGTGTCACCAGTTCAAA	GTTGGATAGGATGGCCCTTG	50.0	55.0	58.1	58.0	754-778 bp	1.0	83.8
trnF-GAA-ndhJ_p3	GGGATAGCTCAGCTGGTAGA	ATCCACGCCTGAAACGTATC	55.0	50.0	58.0	58.1	823-847 bp	1.0	83.5
trnF-GAA-ndhJ_p4	GGGATAGCTCAGCTGGTAGA	AGTTGGATAGGATGGCCCTT	55.0	50.0	58.0	58.1	791-815 bp	1.0	83.4
trnF-GAA-ndhJ_p5	CCTCGTGTCACCAGTTCAAA	ATCCACGCCTGAAACGTATC	50.0	50.0	58.1	58.1	787-811 bp	1.0	83.3