Hotspot

petN-psbM

LSC · 29842-30540 · 699 bp

Back to Heuchera

Pi 0.00986
MarkerSeek score 54.50
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
699
variable_sites
20
pi
0.00986
unique_haplotype_count
11
primer_available
yes
alignment_reliability
0.97
markerseek_score
54.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTCT 50.0 42.9 57.6 57.6 702-803 bp 1.0 72.0
petN-psbM_p2 ATGGGGAAGAAGTGGACTCT TGCTACTGCACTGTTCATTC 50.0 45.0 57.6 56.1 702-803 bp 1.0 67.9
petN-psbM_p3 TGGGGAAGAAGTGGACTCTA TGCTACTGCACTGTTCATTCT 50.0 42.9 57.0 57.6 701-802 bp 1.0 66.7
petN-psbM_p4 ATGGGGAAGAAGTGGACTCT GCTACTGCACTGTTCATTCT 50.0 45.0 57.6 55.8 701-802 bp 1.0 65.5
petN-psbM_p5 AGTATGGGGAAGAAGTGGACT TGCTACTGCACTGTTCATTCT 47.6 42.9 57.8 57.6 705-806 bp 1.0 65.2