Hotspot

atpA-atpF

LSC · 12342-12407 · 66 bp

Back to Heuchera

Pi 0.0175

MarkerSeek score 53.20

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 66
variable_sites: 3
pi: 0.0175
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 53.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	ATGCCATCGCCTACTTGAAG	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.0	58.0	209-214 bp	1.0	82.6
atpA-atpF_p2	ATTGCTCAATACGTTCCCGG	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.3	58.0	151-156 bp	1.0	81.4
atpA-atpF_p3	ATGCCATCGCCTACTTGAAG	ATGTTTGGGGCGATGAAAGA	50.0	45.0	58.0	57.8	210-215 bp	1.0	81.0
atpA-atpF_p4	ATGCCATCGCCTACTTGAAG	GGTATGTTTGGGGCGATGAA	50.0	50.0	58.0	58.2	213-218 bp	1.0	80.7
atpA-atpF_p5	CAATGCCATCGCCTACTTGA	TGTTTGGGGCGATGAAAGAA	50.0	45.0	58.3	58.0	211-216 bp	1.0	80.2