Hotspot

rps16-trnQ-UUG

LSC · 6138-7489 · 1352 bp

Back to Heuchera

Pi 0.0122

MarkerSeek score 61.10

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1352
variable_sites: 47
pi: 0.0122
unique_haplotype_count: 10
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	999-1741 bp	1.0	84.7
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1010-1752 bp	1.0	84.1
rps16-trnQ-UUG_p3	CCACAGCTGATCATGTCCTT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	57.6	58.1	1020-1762 bp	1.0	82.4
rps16-trnQ-UUG_p4	TCCACAGCTGATCATGTCCT	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.4	58.1	1021-1763 bp	1.0	82.4
rps16-trnQ-UUG_p5	AAGTCGCACGTTGCTTTCTA	AGAAAGCTAAATGGGGCGTG	45.0	50.0	58.1	58.5	1058-1800 bp	1.0	80.9