Hotspot

rpoC1

LSC · 21184-23971 · 2788 bp

Back to Heteropolygonatum

Pi 0.00208

MarkerSeek score 57.70

Primer available yes

Label rpoC1

Feature Metadata

label_name: rpoC1
feature_type: gene
region: LSC
length_bp: 2788
variable_sites: 14
pi: 0.00208
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoC1_p1	GGCACCGGTATCGTCTAATC	TTGCTCGTTCGAGAACTACG	55.0	50.0	57.9	58.0	1848-2939 bp	1.0	79.7
rpoC1_p2	GCACCGGTATCGTCTAATCG	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.3	58.0	1847-2938 bp	1.0	77.7
rpoC1_p3	GGCACCGGTATCGTCTAATC	GCTCGTTCGAGAACTACGAT	55.0	50.0	57.9	57.5	1847-2937 bp	1.0	76.4
rpoC1_p4	TTGGATAGACCCGTCTTCCC	TTGCTCGTTCGAGAACTACG	55.0	50.0	58.5	58.0	1871-2962 bp	1.0	76.1
rpoC1_p5	GCACCGGTATCGTCTAATCG	GCTCGTTCGAGAACTACGAT	55.0	50.0	58.3	57.5	1846-2936 bp	1.0	74.4