Hotspot

ccsA

SSC · 115544-116515 · 972 bp

Back to Heteropolygonatum

Pi 0.00391

MarkerSeek score 37.10

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 972
variable_sites: 8
pi: 0.00391
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	GTGAAATCGGTAGACACGCT	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	114-1482 bp	1.0	81.4
ccsA_p2	TGAAATCGGTAGACACGCTG	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	113-1481 bp	1.0	81.4
ccsA_p3	GAGTGGCGGCATACTGTAAA	GGATTCTGGACCACGAGAAC	50.0	55.0	58.0	58.0	113-1422 bp	1.0	81.3
ccsA_p4	GTGAAATCGGTAGACACGCT	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	114-1706 bp	1.0	81.2
ccsA_p5	TGAAATCGGTAGACACGCTG	TTCTCGATGGCTTCTCTTGC	50.0	50.0	58.0	58.0	113-1705 bp	1.0	81.2