Hotspot

ccsA-ndhD

SSC · 116516-116737 · 222 bp

Back to Heteropolygonatum

Pi 0.0279

MarkerSeek score 61.40

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 222
variable_sites: 13
pi: 0.0279
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	CGAATTCGTCACTTGTAGCG	TCGCTATCAGTTGACAAGGT	50.0	45.0	57.3	56.6	112-395 bp	1.0	66.5
ccsA-ndhD_p2	CGAATTCGTCACTTGTAGCG	TTCGCTATCAGTTGACAAGGT	50.0	42.9	57.3	57.3	113-396 bp	1.0	64.4
ccsA-ndhD_p3	ATTCGTCACTTGTAGCGTCT	TCGCTATCAGTTGACAAGGT	45.0	45.0	57.0	56.6	114-392 bp	1.0	63.9
ccsA-ndhD_p4	CGAATTCGTCACTTGTAGCG	TCAGTTGACAAGGTACAAGCT	50.0	42.9	57.3	57.2	113-389 bp	1.0	63.2
ccsA-ndhD_p5	CGAATTCGTCACTTGTAGCG	TTTCGCTATCAGTTGACAAGGT	50.0	40.9	57.3	58.0	114-397 bp	1.0	61.8