Hotspot

psaI-ycf4

LSC · 60385-60732 · 348 bp

Back to Heteropolygonatum

Pi 0.0103

MarkerSeek score 58.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 8
pi: 0.0103
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	GCGATTGCAATGGCTTCTTT	ACTTGCTTTTCGAGACCCTG	45.0	50.0	58.0	58.1	113-462 bp	1.0	80.7
psaI-ycf4_p2	GCGATTGCAATGGCTTCTTT	CTTGCTTTTCGAGACCCTGT	45.0	50.0	58.0	58.1	112-461 bp	1.0	80.7
psaI-ycf4_p3	TTTTGTCCCTTTAGTGGGCC	ACTTGCTTTTCGAGACCCTG	50.0	50.0	58.0	58.1	114-493 bp	1.0	80.6
psaI-ycf4_p4	TTTTGTCCCTTTAGTGGGCC	CTTGCTTTTCGAGACCCTGT	50.0	50.0	58.0	58.1	113-492 bp	1.0	80.6
psaI-ycf4_p5	GTGGGCCTAGTATTTCCTGC	CTTGCTTTTCGAGACCCTGT	55.0	50.0	58.0	58.1	113-479 bp	1.0	80.4