Hotspot

ndhC-trnV-UAC

LSC · 50967-52629 · 1663 bp

Back to Heteropolygonatum

Pi 0.00461

MarkerSeek score 63.20

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 1663
variable_sites: 16
pi: 0.00461
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	GGTCCTTCACTAATCGGAGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.8	57.8	112-1809 bp	1.0	78.7
ndhC-trnV-UAC_p2	GGTCCTTCACTAATCGGAGC	AGCCCAATGCAACAAAATGC	55.0	45.0	57.8	58.5	113-1891 bp	1.0	76.4
ndhC-trnV-UAC_p3	GGTCCTTCACTAATCGGAGC	GAAGGTCTACGGTTCGAGTC	55.0	55.0	57.8	57.5	113-1808 bp	1.0	75.9
ndhC-trnV-UAC_p4	GGTCCTTCACTAATCGGAGC	GTTCGAGTCCGTATAGCCCT	55.0	55.0	57.8	58.7	112-1797 bp	1.0	74.8
ndhC-trnV-UAC_p5	GGTCCTTCACTAATCGGAGC	GAAAGAGCCCAATGCAACAA	55.0	45.0	57.8	57.2	113-1896 bp	1.0	73.6