Hotspot

rpoB-trnC-GCA

LSC · 27220-28473 · 1254 bp

Back to Heteropolygonatum

Pi 0.00686

MarkerSeek score 50.30

Primer available yes

Label rpoB-trnC-GCA

Feature Metadata

label_name: rpoB-trnC-GCA
feature_type: igs
region: LSC
length_bp: 1254
variable_sites: 20
pi: 0.00686
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 50.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpoB-trnC-GCA_p1	CGCCAAGCCCTGATTAATGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.3	57.5	1385-1394 bp	1.0	75.1
rpoB-trnC-GCA_p2	TCCCTCATTTCCATTCCGGA	TAAAGGATTTGCAGTCCCCC	50.0	50.0	58.4	57.5	1313-1322 bp	1.0	74.5
rpoB-trnC-GCA_p3	TTCCCTCATTTCCATTCCGG	TAAAGGATTTGCAGTCCCCC	50.0	50.0	57.6	57.5	1314-1323 bp	1.0	74.3
rpoB-trnC-GCA_p4	CGCCAAGCCCTGATTAATGA	TTTGTTGATCAGGCGACACC	50.0	50.0	58.3	58.8	1422-1431 bp	1.0	73.1
rpoB-trnC-GCA_p5	CGCCAAGCCCTGATTAATGA	GACACCCGGATTTGAACTGG	50.0	55.0	58.3	58.8	1408-1417 bp	1.0	72.6