Hotspot

atpF-atpH

LSC · 13736-14234 · 499 bp

Back to Heteropolygonatum

Pi 0.00241

MarkerSeek score 58.60

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 499
variable_sites: 2
pi: 0.00241
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	ATTAAACCCGAAACTCCCGG	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.9	58.0	621-622 bp	1.0	79.3
atpF-atpH_p2	CAGTGGCCTAAGGAAACGAA	ATTTATGGACTGGTCGTGGC	50.0	50.0	57.8	58.0	593-594 bp	1.0	78.8
atpF-atpH_p3	ATTAAACCCGAAACTCCCGG	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.9	58.3	608-609 bp	1.0	77.4
atpF-atpH_p4	CAGTGGCCTAAGGAAACGAA	TCGTGGCATTAGCGCTTTTA	50.0	45.0	57.8	58.3	580-581 bp	1.0	76.9
atpF-atpH_p5	ATTAAACCCGAAACTCCCGG	CGTGGCATTAGCGCTTTTAT	50.0	45.0	57.9	57.2	607-608 bp	1.0	73.5