Hotspot

trnS-GCU-trnG-UCC

LSC · 8366-9697 · 1332 bp

Back to Heteropolygonatum

Pi 0.00654
MarkerSeek score 60.20
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
1332
variable_sites
18
pi
0.00654
unique_haplotype_count
5
primer_available
yes
alignment_reliability
0.98
markerseek_score
60.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 531-1466 bp 1.0 74.8
trnS-GCU-trnG-UCC_p2 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCACT 50.0 42.9 58.1 58.2 535-1470 bp 1.0 74.8
trnS-GCU-trnG-UCC_p3 ATCAGAGGGAACGGAGAGAG ACGAATCGCACTTTTACCACT 55.0 42.9 57.9 58.2 530-1465 bp 1.0 74.7
trnS-GCU-trnG-UCC_p4 GATCAGAGGGAACGGAGAGA ACGAATCGCACTTTTACCAC 55.0 45.0 57.9 56.8 530-1466 bp 1.0 74.7
trnS-GCU-trnG-UCC_p5 AAGTGATCAGAGGGAACGGA ACGAATCGCACTTTTACCAC 50.0 45.0 58.1 56.8 534-1470 bp 1.0 74.7