Hotspot

psbI-trnS-GCU

LSC · 8163-8277 · 115 bp

Back to Heteropolygonatum

Pi 0.0157

MarkerSeek score 56.30

Primer available yes

Label psbI-trnS-GCU

Feature Metadata

label_name: psbI-trnS-GCU
feature_type: igs
region: LSC
length_bp: 115
variable_sites: 3
pi: 0.0157
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-trnS-GCU_p1	CTTATCTAACGATCCGGGCC	ATTGGGAGAGATGGCTGAGT	55.0	50.0	57.6	58.1	250-254 bp	1.0	77.5
psbI-trnS-GCU_p2	CTTATCTAACGATCCGGGCC	GGGTTCGAATCCCTCTCTCT	55.0	55.0	57.6	58.2	181-185 bp	1.0	77.1
psbI-trnS-GCU_p3	CTTATCTAACGATCCGGGCC	GTGGACTAAAGCGGCAGATT	55.0	50.0	57.6	58.3	232-236 bp	1.0	76.4
psbI-trnS-GCU_p4	CTTATCTAACGATCCGGGCC	TGGACTAAAGCGGCAGATTG	55.0	50.0	57.6	58.3	231-235 bp	1.0	76.3
psbI-trnS-GCU_p5	CTTATCTAACGATCCGGGCC	TTCGAATCCCTCTCTCTCCG	55.0	55.0	57.6	58.3	178-182 bp	1.0	76.3