Hotspot

rps16-trnQ-UUG

LSC · 5713-6992 · 1280 bp

Back to Heteropolygonatum

Pi 0.00485

MarkerSeek score 58.90

Primer available yes

Label rps16-trnQ-UUG

Feature Metadata

label_name: rps16-trnQ-UUG
feature_type: igs
region: LSC
length_bp: 1280
variable_sites: 11
pi: 0.00485
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 58.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps16-trnQ-UUG_p1	AAGTCGCACGTTGCTTTCTA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	58.1	58.1	1242-1353 bp	1.0	83.0
rps16-trnQ-UUG_p2	TCATGTCCTTCAAGTCGCAC	GAGGTTCGAATCCTTCCGTC	50.0	55.0	58.2	58.1	1253-1364 bp	1.0	82.4
rps16-trnQ-UUG_p3	AAGTCGCACGTTGCTTTCTA	TTCGGAGGTTCGAATCCTTC	45.0	50.0	58.1	57.3	1246-1357 bp	1.0	78.2
rps16-trnQ-UUG_p4	AAGTCGCACGTTGCTTTCTA	TAAGGCAACGGGTTTTGGTC	45.0	50.0	58.1	58.7	1272-1383 bp	1.0	78.1
rps16-trnQ-UUG_p5	ATCATGTCCTTCAAGTCGCA	GAGGTTCGAATCCTTCCGTC	45.0	55.0	57.2	58.1	1254-1365 bp	1.0	77.9