Hotspot

rpl16

LSC · 78325-79816 · 1492 bp

Back to Heteropogon

Pi 0.0102

MarkerSeek score 56.20

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1492
variable_sites: 30
pi: 0.0102
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GATTACCAGCGGCTCCTATG	GGGTCGTCTAGCAGGAAAAG	55.0	55.0	57.9	58.0	1978-2016 bp	1.0	82.9
rpl16_p2	TTATACCGTCGTCGCCTTTG	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	58.1	58.0	2116-2154 bp	1.0	82.6
rpl16_p3	TCAGCAATAGTGTCCTTGCC	GGGTCGTCTAGCAGGAAAAG	50.0	55.0	57.9	58.0	2421-2477 bp	1.0	82.1
rpl16_p4	GATTACCAGCGGCTCCTATG	ATCATGTCTTCCCATTCCGC	55.0	50.0	57.9	58.0	1812-1837 bp	0.8	74.8
rpl16_p5	TTATACCGTCGTCGCCTTTG	ATCATGTCTTCCCATTCCGC	50.0	50.0	58.1	58.0	1950-1975 bp	0.8	74.5