Hotspot

rpl32-trnL-UAG

SSC · 105038-105568 · 531 bp

Back to Heteropogon

Pi 0.0298

MarkerSeek score 65.00

Primer available yes

Label rpl32-trnL-UAG

Feature Metadata

label_name: rpl32-trnL-UAG
feature_type: igs
region: SSC
length_bp: 531
variable_sites: 33
pi: 0.0298
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl32-trnL-UAG_p1	AGAAGGATTGATTTGCGGCA	TTCGAACCGAGATGCTTGAG	45.0	50.0	58.2	58.0	842-861 bp	1.0	82.9
rpl32-trnL-UAG_p2	AGAAGGATTGATTTGCGGCA	GGATTCGAACCGAGATGCTT	45.0	50.0	58.2	58.1	845-864 bp	1.0	82.5
rpl32-trnL-UAG_p3	AGAAGGATTGATTTGCGGCA	TCCTAAGAGCAGCGTGTCTA	45.0	50.0	58.2	57.9	815-834 bp	1.0	82.1
rpl32-trnL-UAG_p4	AGAAGGATTGATTTGCGGCA	ATGTCCCGAAGAAACCCAAC	45.0	50.0	58.2	58.1	1128-1140 bp	1.0	81.8
rpl32-trnL-UAG_p5	AGAAGGATTGATTTGCGGCA	GATCCGCACAAAAGAGTTGC	45.0	50.0	58.2	58.0	1394-1406 bp	1.0	81.7