Hotspot

ndhF-rpl32

SSC · 103989-104857 · 869 bp

Back to Heteropogon

Pi 0.0151

MarkerSeek score 62.00

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 869
variable_sites: 25
pi: 0.0151
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	CTGGAAGTGGGAGAAGAGGA	TGACATCGAAGTACGTTTTT	55.0	35.0	58.1	53.2	909-954 bp	1.0	47.6
ndhF-rpl32_p2	CTGGAAGTGGGAGAAGAGGA	TTGACATCGAAGTACGTTTT	55.0	35.0	58.1	53.2	910-955 bp	1.0	47.6
ndhF-rpl32_p3	CTGGAAGTGGGAGAAGAGGA	TTTGACATCGAAGTACGTTT	55.0	35.0	58.1	53.2	911-956 bp	1.0	47.6
ndhF-rpl32_p4	CTGGAAGTGGGAGAAGAGGA	TTTTGACATCGAAGTACGTT	55.0	35.0	58.1	53.2	912-957 bp	1.0	47.6
ndhF-rpl32_p5	CTGGAAGTGGGAGAAGAGGA	TTTTTGACATCGAAGTACGT	55.0	35.0	58.1	53.2	913-958 bp	1.0	47.5