Hotspot

trnL-UAA-trnF-GAA

LSC · 48227-48600 · 374 bp

Back to Heteropogon

Pi 0.0166

MarkerSeek score 64.70

Primer available yes

Label trnL-UAA-trnF-GAA

Feature Metadata

label_name: trnL-UAA-trnF-GAA
feature_type: igs
region: LSC
length_bp: 374
variable_sites: 12
pi: 0.0166
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 64.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAA-trnF-GAA_p1	AAGTCGTGAGGGTTCAAGTC	TTTGAACTGGTGACACGAGG	50.0	50.0	57.5	58.1	448-478 bp	1.0	80.6
trnL-UAA-trnF-GAA_p2	AAGTCGTGAGGGTTCAAGTC	CTGTGCCAGGAACCAGATTT	50.0	50.0	57.5	58.1	465-495 bp	1.0	80.1
trnL-UAA-trnF-GAA_p3	AAGTCGTGAGGGTTCAAGTC	GTGCCAGGAACCAGATTTGA	50.0	50.0	57.5	58.1	463-493 bp	1.0	80.1
trnL-UAA-trnF-GAA_p4	AAGTCGTGAGGGTTCAAGTC	TTTTTCTGTGCCAGGAACCA	50.0	45.0	57.5	57.8	470-500 bp	1.0	79.5
trnL-UAA-trnF-GAA_p5	AAGTCGTGAGGGTTCAAGTC	TTTTCTGTGCCAGGAACCAG	50.0	50.0	57.5	58.3	469-499 bp	1.0	78.3