Hotspot

atpF-atpA

LSC · 35276-35366 · 91 bp

Back to Heteropogon

Pi 0.022

MarkerSeek score 67.20

Primer available yes

Label atpF-atpA

Feature Metadata

label_name: atpF-atpA
feature_type: igs
region: LSC
length_bp: 91
variable_sites: 4
pi: 0.022
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpA_p1	ATTTCCGTACGATTCGTGCT	TTGTTCAATACGTTCGCGGA	45.0	45.0	57.7	58.2	205-207 bp	1.0	78.3
atpF-atpA_p2	CATTTCCGTACGATTCGTGC	TTGTTCAATACGTTCGCGGA	50.0	45.0	57.7	58.2	206-208 bp	1.0	77.8
atpF-atpA_p3	AATATTGGCATTCTCGGGGC	TTGTTCAATACGTTCGCGGA	50.0	45.0	58.4	58.2	185-187 bp	1.0	77.8
atpF-atpA_p4	ATTTCCGTACGATTCGTGCT	TTCAATACGTTCGCGGAGAA	45.0	45.0	57.7	57.6	202-204 bp	1.0	77.2
atpF-atpA_p5	ATTTCCGTACGATTCGTGCT	GTTCAATACGTTCGCGGAGA	45.0	50.0	57.7	58.4	203-205 bp	1.0	77.0