Hotspot

trnC-GCA-rpoB

LSC · 19070-20276 · 1207 bp

Back to Heteropogon

Pi 0.0141

MarkerSeek score 57.70

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 1207
variable_sites: 34
pi: 0.0141
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	AAAGGATTTGCAGTCCCCTG	CCCTCATTTCCATTCCGGA	50.0	52.6	58.1	56.8	1266-1336 bp	1.0	65.4
trnC-GCA-rpoB_p2	CTGGGGGTAAAGGATTTGCA	CCCTCATTTCCATTCCGGA	50.0	52.6	57.8	56.8	1274-1344 bp	1.0	64.1
trnC-GCA-rpoB_p3	TGGGGGTAAAGGATTTGCAG	CCCTCATTTCCATTCCGGA	50.0	52.6	57.8	56.8	1273-1343 bp	1.0	64.1
trnC-GCA-rpoB_p4	ACTGGGGGTAAAGGATTTGC	CCCTCATTTCCATTCCGGA	50.0	52.6	57.8	56.8	1275-1345 bp	1.0	64.1
trnC-GCA-rpoB_p5	GGGGGTAAAGGATTTGCAGT	CCCTCATTTCCATTCCGGA	50.0	52.6	57.8	56.8	1272-1342 bp	1.0	64.1