Hotspot

trnD-GUC-psbM

LSC · 16652-17716 · 1065 bp

Back to Heteropogon

Pi 0.0101

MarkerSeek score 61.80

Primer available yes

Label trnD-GUC-psbM

Feature Metadata

label_name: trnD-GUC-psbM
feature_type: igs
region: LSC
length_bp: 1065
variable_sites: 24
pi: 0.0101
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 61.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnD-GUC-psbM_p1	AATAGGCATGCCATACACCC	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	57.7	57.9	1200-1232 bp	1.0	56.2
trnD-GUC-psbM_p2	TTCAATTGGTCAGAGCACCG	GGCAGTAGGAACTAGAATGAACA	50.0	43.5	58.5	57.9	1166-1198 bp	1.0	54.8
trnD-GUC-psbM_p3	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGAAC	50.0	43.5	57.7	57.7	1201-1233 bp	1.0	54.1
trnD-GUC-psbM_p4	AATAGGCATGCCATACACCC	AGGCAGTAGGAACTAGAATGA	50.0	42.9	57.7	55.5	1201-1233 bp	1.0	52.9
trnD-GUC-psbM_p5	AATAGGCATGCCATACACCC	TGCGAGAATATTGACTTCCA	50.0	40.0	57.7	54.5	1161-1193 bp	1.0	52.8