Hotspot

rpl16

LSC · 82697-84114 · 1418 bp

Back to Heteroblemma

Pi 0.0227

MarkerSeek score 58.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: LSC
length_bp: 1418
variable_sites: 32
pi: 0.0227
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 58.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	ATGCCGTTGTTGCGTTTAAG	GAGGGAATTGCACGCATAGA	45.0	50.0	58.0	58.0	2427-2432 bp	1.0	80.9
rpl16_p2	TCTCAATTCTCGGGCAATCG	GAGGGAATTGCACGCATAGA	50.0	50.0	58.1	58.0	2524-2529 bp	1.0	80.7
rpl16_p3	CATCCGTAATCACAGCGACA	GAGGGAATTGCACGCATAGA	50.0	50.0	58.1	58.0	2339-2344 bp	1.0	80.7
rpl16_p4	ATCGGCGATTACTAGCTCCT	GAGGGAATTGCACGCATAGA	50.0	50.0	58.1	58.0	2297-2302 bp	1.0	80.6
rpl16_p5	CTGTCTCAATTCTCGGGCAA	GAGGGAATTGCACGCATAGA	50.0	50.0	57.9	58.0	2527-2532 bp	1.0	80.4