Hotspot

ndhF-rpl32

SSC · 114687-115576 · 890 bp

Back to Heteroblemma

Pi 0.0407
MarkerSeek score 72.50
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
890
variable_sites
36
pi
0.0407
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 TGGGAAGTGGAATGAGAGGT CCTTCAATGCTGTAAAATAGGCT 50.0 39.1 58.0 57.2 1033-1065 bp 1.0 52.0
ndhF-rpl32_p2 TGGGAAGTGGAATGAGAGGT CCTTCAATGCTGTAAAATAGGCTT 50.0 37.5 58.0 57.8 1033-1065 bp 1.0 49.1
ndhF-rpl32_p3 TGGGAAGTGGAATGAGAGGT CCTTCAATGCTGTAAAATAGGC 50.0 40.9 58.0 55.8 1033-1065 bp 1.0 48.8
ndhF-rpl32_p4 TGGGAAGTGGAATGAGAGGTA CCTTCAATGCTGTAAAATAGGCT 47.6 39.1 57.8 57.2 1033-1065 bp 1.0 43.1
ndhF-rpl32_p5 ATGGGAAGTGGAATGAGAGG CCTTCAATGCTGTAAAATAGGCT 50.0 39.1 56.6 57.2 1034-1066 bp 1.0 43.1