Hotspot

psbH-petB

LSC · 76177-76313 · 137 bp

Back to Heteroblemma

Pi 0.0438

MarkerSeek score 77.70

Primer available yes

Label psbH-petB

Feature Metadata

label_name: psbH-petB
feature_type: igs
region: LSC
length_bp: 137
variable_sites: 6
pi: 0.0438
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbH-petB_p1	TCCTTTGATGGGTGTTGCAA	TCCGTGCTCCAGATACTAGA	45.0	50.0	57.9	57.0	355 bp	1.0	72.5
psbH-petB_p2	GGAACCACTCCTTTGATGGG	TCCGTGCTCCAGATACTAGA	55.0	50.0	58.2	57.0	363 bp	1.0	71.9
psbH-petB_p3	GGGTGTTGCAATGGCTCTAT	TCCGTGCTCCAGATACTAGA	50.0	50.0	58.2	57.0	346 bp	1.0	71.3
psbH-petB_p4	AACCACTCCTTTGATGGGTG	TCCGTGCTCCAGATACTAGA	50.0	50.0	57.7	57.0	361 bp	1.0	70.8
psbH-petB_p5	CCACTCCTTTGATGGGTGTT	TCCGTGCTCCAGATACTAGA	50.0	50.0	57.7	57.0	359 bp	1.0	70.8