Hotspot

rps12-clpP1

LSC · 71074-71249 · 176 bp

Back to Heteroblemma

Pi 0.071

MarkerSeek score 75.00

Primer available yes

Label rps12-clpP1

Feature Metadata

label_name: rps12-clpP1
feature_type: igs
region: LSC
length_bp: 176
variable_sites: 12
pi: 0.071
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps12-clpP1_p1	AATAAGTTGTTTAATAGTTGGCA	GCCCAAGCTCATGGAATTGT	26.1	50.0	52.6	58.8	242-271 bp	1.0	45.9
rps12-clpP1_p2	ATAAGTTGTTTAATAGTTGGCAT	GCCCAAGCTCATGGAATTGT	26.1	50.0	52.4	58.8	241-270 bp	1.0	45.9
rps12-clpP1_p3	AATAAGTTGTTTAATAGTTGGCAT	GCCCAAGCTCATGGAATTGT	25.0	50.0	53.2	58.8	242-271 bp	1.0	45.9
rps12-clpP1_p4	TCTAATAAGTTGTTTAATAGTTGGCA	GCCCAAGCTCATGGAATTGT	26.9	50.0	55.2	58.8	245-274 bp	1.0	45.9
rps12-clpP1_p5	AATAAGTTGTTTAATAGTTGGCA	CCCAAGCTCATGGAATTGTTG	26.1	47.6	52.6	57.8	241-270 bp	1.0	45.9