Hotspot

rpl33-rps18

LSC · 68887-69106 · 220 bp

Back to Heteroblemma

Pi 0.0556

MarkerSeek score 72.90

Primer available yes

Label rpl33-rps18

Feature Metadata

label_name: rpl33-rps18
feature_type: igs
region: LSC
length_bp: 220
variable_sites: 12
pi: 0.0556
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl33-rps18_p1	CGATTCACGGGGAGATAAAGA	TCAATTCGATCCCCCGATTG	47.6	50.0	57.3	57.7	328-332 bp	1.0	68.0
rpl33-rps18_p2	CGATTCACGGGGAGATAAAGAA	TCAATTCGATCCCCCGATTG	45.5	50.0	57.9	57.7	328-332 bp	1.0	65.4
rpl33-rps18_p3	CGATTCACGGGGAGATAAAGA	ATTCGATCCCCCGATTGGAT	47.6	50.0	57.3	58.6	325-329 bp	1.0	65.0
rpl33-rps18_p4	CGATTCACGGGGAGATAAAGA	CAATTCGATCCCCCGATTGG	47.6	55.0	57.3	58.8	327-331 bp	1.0	64.0
rpl33-rps18_p5	CGATTCACGGGGAGATAAAG	TCAATTCGATCCCCCGATTG	50.0	50.0	55.7	57.7	328-332 bp	1.0	63.8