Hotspot

ndhC-trnV-UAC

LSC · 51507-52224 · 718 bp

Back to Heteroblemma

Pi 0.0226
MarkerSeek score 69.90
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
718
variable_sites
16
pi
0.0226
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.99
markerseek_score
69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GGACCTTTACTAATCGGGGC GGTTCGAATCCGTATAGCCC 55.0 55.0 57.7 57.9 853-883 bp 1.0 79.8
ndhC-trnV-UAC_p2 AATCGGGGCTAAAATTCCGG GGTTCGAATCCGTATAGCCC 50.0 55.0 58.3 57.9 842-872 bp 1.0 79.3
ndhC-trnV-UAC_p3 GGACCTTTACTAATCGGGGC CGGTTCGAATCCGTATAGCC 55.0 55.0 57.7 58.3 854-884 bp 1.0 77.9
ndhC-trnV-UAC_p4 CGGGGCTAAAATTCCGGAAA GGTTCGAATCCGTATAGCCC 50.0 55.0 58.5 57.9 839-869 bp 1.0 77.6
ndhC-trnV-UAC_p5 AATCGGGGCTAAAATTCCGG CGGTTCGAATCCGTATAGCC 50.0 55.0 58.3 58.3 843-873 bp 1.0 77.5