Hotspot

trnE-UUC-trnT-GGU

LSC · 31208-32189 · 982 bp

Back to Heteroblemma

Pi 0.0359

MarkerSeek score 66.80

Primer available yes

Label trnE-UUC-trnT-GGU

Feature Metadata

label_name: trnE-UUC-trnT-GGU
feature_type: igs
region: LSC
length_bp: 982
variable_sites: 28
pi: 0.0359
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.79
markerseek_score: 66.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnE-UUC-trnT-GGU_p1	TTTCGTAGTACCCTACCCCC	CCATGGCGTTACTCTACCAC	55.0	55.0	57.8	58.1	906-1102 bp	1.0	84.4
trnE-UUC-trnT-GGU_p2	TTTCGTAGTACCCTACCCCC	TGGCGTTACTCTACCACTGA	55.0	50.0	57.8	58.1	903-1099 bp	1.0	84.3
trnE-UUC-trnT-GGU_p3	TGTCCTGAACCACTAGACGA	CCATGGCGTTACTCTACCAC	50.0	55.0	57.7	58.1	847-1043 bp	1.0	83.6
trnE-UUC-trnT-GGU_p4	TGTCCTGAACCACTAGACGA	TGGCGTTACTCTACCACTGA	50.0	50.0	57.7	58.1	844-1040 bp	1.0	83.4
trnE-UUC-trnT-GGU_p5	TTTCGTAGTACCCTACCCCC	ACCATGGCGTTACTCTACCA	55.0	50.0	57.8	58.4	907-1103 bp	1.0	81.5