Hotspot

psbM-trnD-GUC

LSC · 29936-30670 · 735 bp

Back to Heteroblemma

Pi 0.0286

MarkerSeek score 69.40

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 735
variable_sites: 21
pi: 0.0286
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 69.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	TCACTTTGACTCACGGTTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.4	58.6	899-904 bp	1.0	57.2
psbM-trnD-GUC_p2	TCACTTTGACTCACGGTTTTT	TCAATTGGTTAGAGCACCGC	38.1	50.0	56.2	58.6	899-904 bp	1.0	55.6
psbM-trnD-GUC_p3	AATCACTTTGACTCACGGTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	901-906 bp	1.0	55.3
psbM-trnD-GUC_p4	ATCACTTTGACTCACGGTTT	TCAATTGGTTAGAGCACCGC	40.0	50.0	55.2	58.6	900-905 bp	1.0	55.3
psbM-trnD-GUC_p5	AGAATGAAGAGTGCAGTAGC	TCAATTGGTTAGAGCACCGC	45.0	50.0	55.2	58.6	845-850 bp	1.0	55.2