Hotspot

trnS-GCU-trnR-UCU

LSC · 7910-9717 · 1808 bp

Back to Heteroblemma

Pi 0.0189
MarkerSeek score 67.10
Primer available yes
Label trnS-GCU-trnR-UCU

Feature Metadata

label_name
trnS-GCU-trnR-UCU
feature_type
igs
region
LSC
length_bp
1808
variable_sites
34
pi
0.0189
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
67.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnR-UCU_p1 CAATCCGACGCTTTAGTCCA AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1921-1945 bp 1.0 70.2
trnS-GCU-trnR-UCU_p2 AATCCGACGCTTTAGTCCAC AGAACTTGCGTCCAATAGGA 50.0 45.0 58.0 56.6 1920-1944 bp 1.0 70.2
trnS-GCU-trnR-UCU_p3 CAATCCGACGCTTTAGTCCA ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1928-1952 bp 1.0 68.3
trnS-GCU-trnR-UCU_p4 AATCCGACGCTTTAGTCCAC ACATAGAAGAACTTGCGTCCA 50.0 42.9 58.0 57.3 1927-1951 bp 1.0 68.3
trnS-GCU-trnR-UCU_p5 CAATCCGACGCTTTAGTCCA AAGAACTTGCGTCCAATAGGA 50.0 42.9 58.0 57.3 1922-1946 bp 1.0 68.2